Polymerase chain reaction and restriction fragment length polymorphism of cytocrome oxidase subunit I used for differentiation of Brazilian Biomphalaria species intermediate host of Schistosoma mansoni.
نویسندگان
چکیده
The intermediate hosts of Schistosoma mansoni, in Brazil, Biomphalaria glabrata, B. tenagophila and B. straminea, were identified by restriction fragment length polymorphism analysis of the mitochondrial gene cytochrome oxidase I (COI). We performed digestions with two enzymes (AluI and RsaI), previously selected, based on sequences available in Genbank. The profiles obtained with RsaI showed to be the most informative once they were polymorphic patterns, corroborating with much morphological data. In addition, we performed COI digestion of B. straminea snails from Uruguay and Argentina.
منابع مشابه
Polymerase chain reaction and restriction fragment length polymorphism analysis of the ITS2 region for differentiation of Brazilian Biomphalaria intermediate hosts of the Schistosoma mansoni.
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ورودعنوان ژورنال:
- Memorias do Instituto Oswaldo Cruz
دوره 97 Suppl 1 شماره
صفحات -
تاریخ انتشار 2002